However, even if the degassing system works properly, some air bubbles might stick in solvent lines or U/HPLC system fluidics. Preparation steps: Flush the pump head with an appropriate solvent using the purge function. Use only HPLC-grade solvents and water filtered through 0. So for example, if you normally run at 100 bar and the pressure drops to 5 bar, then the pump will shut down. The 1260 Infinity II Flexible Pump is a quaternary UHPLC pump that provides gradient formation and solvent blending from up to four solvents. Check for leaks and perform PM. Draw approximately 20 mL of fluid. I usually let the column drip into a beaker while washing it. Search by lot number or partial lot number. between the degasser and the column. Loaded 0%. 4. Overlay of 6 pump proportioning chromatograms in 10% gradient. Replacing a Check Valve Cartridge. This video demonstrates the proper procedure to manually prime the pump on a Shimadzu LC 40 instrument. Prepare the Pump • Use fresh or different mobile phase (as required). Open the manual purge valve or use the purge command, depending on the pump type. Start pump purging (all channels, including those not used for the application) and wait until pump purging is complete; Flush the entire chromatography system with the starting solvent of your application at a reasonable flow rate to rinse out any other solvent; Install the appropriate HPLC column for your application; Close all doors of the. It accommodates flow rates from 0. Purge pump at high flow rate (e. 결과 및 정리. This sets the mobile phase composition to 100% water so we can purge the water line. Run at high flow: Next, Dolan advises to run the pump for several minutes at high flow (e. The target url is a user manual for the Agilent 1290 Infinity Quaternary Pump G4204A, a high-performance liquid chromatography (HPLC) device that can handle a wide range of flow rates and compositions. This is needed to purge any air from the tubing, introduce fresh mobile phase to each line and then to VERIFY that each channel delivers the reported amount of fluid to the column (measure it). In the right part of the image, the glass piston is. This will redirect the solvent flow to the waste exit of the valve instead of passing through the. e. 05 min is normal however the behavior of the method should be used to determine what is a normal variation. When they fill with air, they lose their prime and stop pumping. Initial System inspection It is always worthwhile to spend a few minutes before starting analysis on assessing the system suitability for the analysis task in hand. Abstract and Figures. You. Fig. 0 • August 2022 *4820. Pump head assemblies Draw in and deliver eluent. . A purge valve including a PTFE frit is fitted at the pump outlet for convenient priming of the pump head. The reduction in condensation risk can allow the pump to achieve its vapor duty at. Low delay volume, extended pressure range up to 800 bar, analytical flow rates up to 5. ), prime system if necessary. You may need to remove and sonicate it. Enter terms to search videos. If problem persists, flush system with 100% methanol or isopropanol. An autosampler works by aspirating and injecting a precise sample volume into the mobile phase stream. Turn on the pump flow rate to 3 mL/min through your CDS Software, Lab Advisor or through the Instant Pilot and open. See "Pressure pulsation or inconstant pressure" in Pressure on page 120. 2HPLC is a High Performance liquid Chromatography. P680 HPLC PumpsPurge pump at high flow rate (e. Non-circular drive gears provide smooth, uniform eluent delivery. ) The easy to use and economically designed LC-40D pump enables reliable HPLC chromatography in isocratic, binary, or LPGE modes. Peaks Peak area precision Pressure Retention times Baseline Troubleshooting overview Running into issues with your HPLC or UHPLC analysis?8th May 2023 HPLC Pump Care and Troubleshooting Many of the technical support inquiries we receive at Chrom Tech are related to care and troubleshooting of our. Set the pump to 100% 'A' at 1. 0*Steps to follow: To backflush the needle seat according to the following procedure, you need a pump with a manual purge valve. 02 to 0. ID stainless steel tubing kit • Galaxie Chromatography Software-0. 7. The 1260 Infinity II Flexible Pump combines high performance and simplified operation with highest flexibility in automated gradient formation and solvent blending. The system will start the countdown from 5 minutes to zero. We routinely see HPLC systems with white fluffy crystals built up around the pump heads, pistons and various fittings from lack of maintenance on a daily basis. It offers gradient formation from up to four solvents at pressures up to 1300 bar and flow rates up to 5 mL/min. 6:51. A pressure rating of up to 400 bar and chemical resistance to a wide. The technical specifications of all pumps meet the highest. Purge valve open. More lab efficiency with your auto delivery schedule, modify and cancel it. Feeding the degasser with an inferior mobile phase will create inferior results. GasesUltiMate 3000 Series: Pump Series Operating Instructions Page I Declaration of Conformity (Original Declaration) Product: UltiMate 3000 Series Pump Types: LPG-3400AB, LPG-3400M and LPG-3400MB DGP-3600A, DGP-3600AB,. The stationary phase is a granular material with very small porous particles in a separation column. 15 mL/min. Passivation is a metal finishing process to prevent corrosion and resist rust. Reassemble pump head and reinstall. For more mobile phase flexibility in any ultrahigh speed and ultrahigh resolution application, the Thermo Scientific™ Dionex™ Ultimate™ HPG-3400RS Rapid Separation Binary Pump offers more solvent capability. This video shows the operation a HPLC purge valve. Purge pump at high flow rate (e. For long-term storage, it is best to follow manufacturer’s recommendations. We have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. Pump 15 minutes at this pressure to wear in the seals. If the column was used with buffered. For more information, visit tutorial demonstrates how to purge the system with Bio-Rad's new NGC liquid chromatography. Purging has only helped to reduce. Currently loaded videos. Choose between 3 configurations: A 2 × 2 binary high pressure gradient (HPG) for high accuracy blending of up to two eluents from two selectable solvents each. If a purge valve is not functioning correctly, it can lead to the vehicle losing power or stalling unexpectedly. Honoring the Past, Focusing on the Future: A Conversation with Jess Lee. • Purge each channe lwtih 25–3m. Priming and Purging the 1525 Binary HPLC Pump. Prepare the Sampler • Avoid using needle wash and seat backflush with organic solvents with buffer applications,Most solvents need to be degassed, so U/HPLC systems typically include a solvent degassing system. For best performance you must know your eluents 2. Vacuum degassing: the solvent is exposed to a vacuum and the reduced pressure removes more than 60% of the dissolved air. HPLC maintenance tutorial on how to change the High Pressure Filter on an Agilent. 1 Procedure for Operation of HPLC pump: • Ensure that all the cables and power cords of the pumps are connected properly. Pump 15 min at this pressure to wear the seals in. 5 um frit for 1. 2. 000 ml, – 25 psi. Initial investigation should consist of purging the pump with fresh eluent to confirm no air is present within the pump, as the presence of air within the pump may cause fluctuations in pressure. Versatile and Flexible The Thermo Scientific™ Dionex™ UltiMate 3000 pump family offers the most complete choice in the industry. 5:26. The good news is that there is a simple solution: open the purge valve, turn the flow rate to five mLs a minute, let it sit there for 10 to. Liquid Chromatography (LC) Troubleshooting Resources. See your pump modules user manual for detailed instructions on re-assembly. , 5-10 mL/min. 06% or 0. Draw. Open the purge valve on the Agilent Infinity 1260 isocratic pump b. Perform 2 purge cycles. Prime the pump with degassed solvent. Run through the prime purge valve first (10 mls), then. Cause a:Air bubble in pump head. Perform seal wear-in procedure. BEFORE WORKING WITH THIS ANALYTICAL HPLC 1. Need to change the pump seal on your Shimadzu LC-20AD HPLC? Boom, I got you. 7. This technique is commonly used in labs for the purpose of separating, identifying, and quantifying components in a mixture. Algae Growth in HPLC Systems 53 5 Optimizing Performance 55. Purification Sample Management From HPLC fraction collection and aspiration to sample injection, Waters provides easy-to-use sample management options. 17: Ready Check Results window appear s after the NC pump blocks purge is executed 2. Draw syringe back to prime. Click the [Auto Purge] button to perform auto purge. 45 or 0. 3. Contacting Us Vanquish Pumps (VH-P10) Operating Manual Page 3 Contacting Us There are several ways to contact us: Ordering Information For ordering information or sales. you will need to • General cleaning Daily first re-prime the pump by purging the air from all the lines. Turn off the pump flow and wait until the system pressure is down to zero. Second, the dissolved gasses must diffuse through the membrane (flat film or tubing wall). 1 ml/min and connect the size exclusion column (4. With regular pump usage, this is recommended every six months. If the pressure does not rise, the purge valve needs to be replaced as it was the cause of high pressure. - filter non-HPLC grade solvents - filter buffer solutions - Install an in-line filter between auto-sampler and column (removes pump seal debris, ALS rotor debris, and sample particulates). This pump also facilitates seamless method transfer using ISET and automated buffer blending. Between samples when you are working with new compounds. Operating principle. If only an occasional bubble is present, the flow rate will be erratic, causing retention time problems. It is by far the number one cause of problems in HPLC. off. Solvent Degasser – removes air gases from the solvents as they flow to the HPLC pump. Purge Valve Assembly 115 Active Inlet Valve Assembly 116 Accessory Kit G1311-68705 117. Replace the filter frits if necessary. You may use the. The FRC-40 features an open-access design, a parameter simulation to ensure pure fractions, compatibility with many racks, and up to 4-channel acquisition. 1 Turn on LC20AD (component 4) and SIL20A (component 3). When autosampler inject, HPLC pressure pump falls down and rise again. Solving Problems That Matter - Bioprocessing. Leak at pump 18 Selectivity Differences in selectivity 19 Baseline Disturbance at void time 20. Vacuum degassing removes more than 60% of dissolved air. If it does not, check the state of the Solvent Filters and replace them. *Run to run RT variation of ±0. For reverse-phase columns, wet the column using a mobile phase like methanol and bring it to normal flow rate. Honoring the Past, Focusing on the Future: A Conversation with Jess Lee. e. Yes it looks like this! Now the valve is ok and there’s no more leak, maybe now the frit is soaked by the mobile phase and fits better?! Thanks!! reply from dmarchini< dmarchini >…. 0. 22μm porosity membrane filter. Figure 7: Pump tab. After waiting a specified time, it starts pumping the mobile phase at the flow rate specified in the analytical method to automatically equilibrate the. Secondary Pump Head Parts 216 Purge Valve 220 Cover Parts 222 Leak Parts 224 Fuses 224 Accessory Kit 225 Tools 226 HPLC System Tool Kit 228. And, remember, if you have a dual-piston pump, you have two piston seals to replace. BTW: I find it useful to tip the flat bottomed solvent. If mobile-phase outgassing occurs within the LC system, the most common problem areas are the pumps and detector. Newsroom; Company Information;HPLC Setup Section 6. Connect its other end to a waste container. you will need to • General cleaning Daily first re-prime the pump by purging the air from all the lines. Prime the HPLC pumps under the following conditions: - starting the pumps for the first time after it has been idle for more than 24 hours. g. The historical evolution of HPLC pumps and their characteristics has been reviewed in many textbooks (1–3), as well as journal articles (4–6). Request a Certificate. HPLC pumps are designed to pump liquid, not – Improve Peak Shape for Ionizable Compounds air. 5 ml/min for 4 min, I only collected 1 ml of liquid. It is Liquid Chromatography. 88 Service Handbook for 1050 Series of HPLC Modules - 11/2001 Pumps: General Information Specifications Specifications Table 2 Specifications of 1050 PumpsHi,when I do wet prime/dry prime in hplc ? Thank you. If problem persists, flush system with 100% methanol or isopropanol. Purge pump and attempt to clean check valves. It can mainly divided by two types 1. Rough pumps stay on so the system remains under vacuum. Relevant functions marked for purging, flow settings ect. 결과 및 정리. The pump assembly includes a pump head with an active inlet valve which has an replaceable cartridge, and an outlet valve. For a system in reversed phase -> Purge all lines with hot HPLC-grade water (60 to 70 °C) to remove salts or ACN polymeric by-products. 1:55. The system guide, Waters doc# 71500269502, details. This technique is commonly used in labs for the purpose of separating, identifying, and quantifying components in a mixture. Flow Connections of the Binary Pump with Solvent Selection Valve 24 Flow Connections of the Binary Pump without Solvent Selection Valve 27 Priming and Purging the System 30 2 Optimizing Performance How to optimize the binary pump to achieve best chromato-graphic results 33 Hints for Successful Use of the Binary Pump 34 Solvent. OBJECTIVE: To clean and flush an Alliance HPLC System or an ACQUITY UPLC system to remove contaminants. Flush the pump head with an appropriate solvent using the purge function; Turn off the pump flow and wait until the system pressure is down to zero; Analysis. After purging is done click [Pump on] button / to deliver the solvent for at least 20 to 30min to rinse the flow line . Problem 3:Inconsistent or too slow pump flow rate. Agilent University. If the pressure reaches its maximum (press. After the valve sonication procedure, it is suggested to purge the pump with water at 60 degrees, keeping the purge valve open. - For Quick Start: Click the "Press to make a single injection" button in the Acquisition. The display will show messages ‘Start PURGE’ and ‘Press ENTER to confirm’. A damping unit is connected between the two plunger chambers. Press the purge buttons again to. Flush the pump with 100% filtered, distilled water for at least 3 minutes. Remove and clean pistons. (Prime each pump head separately. 1. Replacing the Plunger Seal and Plunger. Prime the HPLC pumps under the following conditions: - starting the pumps for the first time after it has been idle for more than 24 hours. Clog removal. Hello, I have dual pump RSLC dionex 3000. , 5-10 mL/min. The source of column clogging comes from particulates in a number of places:We have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. Prepare the solvent: Begin by ensuring that the solvent is filtered and degassed. Before using an HPLC system, you should prime all of the lines in your HPLC pump. Flush the system. Almost all descriptions in the manual apply to all pump models in the LPG-3000 pump series and cover both the standard (stainless steel) and biocompatible pumps. 11 Click everything on. A flow rate of 5mL/min is commonly used for standard HPLC systems. Click on Method 1. Therefore, the term "thePurge pump and attempt to clean check valves. Check for signs of leaks. 5. When I used the pump C last week the pressure suddenly fell down from 300 PSI to 0 (with 100% methanol, 0. It is recommended to change your HPLC pump’s piston seals during planned preventative maintenance. . max) right away, reduce the flowrate before checking. Pump 100% isopropanol through the pump for 3 minutes. Turn off the pump by pressing "Pump" on the LC-20AD 4. Program the pump to purge the column with strong solvents. The system will start the countdown from 5 minutes to zero. High Pressure Liquid Chromatography. Push the “PURGE” button on the front of the pump. 1260 Infinity Binary Pump User Manual 11 Introduction to the Binary Pump 1 Instrument and OperationThe pump assembly includes a pump head with a passive inlet valve and an outlet valve. It is recommended to change your HPLC pump’s piston seals during planned preventative maintenance. Pump – Open the purge valve and purge every solvent channel with 5 mL/min for 5 minutes to remove bubbles in your solvent lines (Note: if the backpressure at purging is higher than 10 bar, please replace the PTFE frit in the purge valve) – Make sure that the flushing solvent is mixable with the storage solvent of your solvent channel. Prepare the Sampler • Avoid using needle wash and seat backflush with organic solvents with buffer applications, Most solvents need to be degassed, so U/HPLC systems typically include a solvent degassing system. 2 Push the “conc” button on LC20AD. When purging on LC-20AD is done, press the "Purge" bottom on SIL. Column frits are a common point of clogging. Maintaining Your Agilent 1100 Series HPLC System Optimize performance and maximize efficiency in your lab. )• Operation Purging the Pump Purging the pump means rinsing the pump for a short time at a higher flow rate, using the selected solvents. solvents. The mobile phase, on the other hand, is a solvent or solvent mixture which is forced at high pressure through the separation column. The manual is in English and can be downloaded as a PDF file. For additional troubleshooting help try downloading the Thermo Scientific HPLC Troubleshooting Guide. It also includes relevant guidance for practical handling and mainte-(1:27min) Exchanging the Purge Valve Frit or the Purge Valve (3. Power off the pump and remove the waste tubing from the purge valve. Channels in column Replace column. 2 Wellplate Autosampler Purge Kit Features Features The new Wellplate Autosampler Purge Kit offers the following features: • Fits for Agilent 1100 Series Wellplate Autosampler (G1367A and G1368A (thermostatted)). The 1290 Infinity II High Speed Pump uses high-pressure mixing for UHPLC gradient formation and solvent blending from up to two solvents at pressures up to 1300 bar and flow rates up to 5 mL/min. From my little experience i learnt that it may be due to air trapped in. For modular autosamplers purge from front panel. Up to 6 solvent channels for binary high-pressure mixing. 1 Turn on LC20AD (component 4) and SIL20A (component 3). Set flow to 0ml/min f. HPLC is an abbreviation for High Performance Liquid Chromatography. The pressure does not rise. The pump offers a dynamic flow rate range from 1 to 50 mL/min at up to 420 bar, allowing workflows on. If problem still persists, contact system manufacturer. If you cannot see a leak at the bottom of the pump head when you try to purge, then most likely a leak is not the cause of the issue. Prepare the Pump • Use fresh or different mobile phase (as required). 8. close the purge. Two of the most widely used methods to degas a mobile phase are: Helium purging: helium is bubbled through the solvent and removes up to 80% of dissolved air. Peak shape C. Initially, the pressure was unstable. The impact of this type of clogging will be gradual, not sudden. The system must be primed to remove air bubbles and for the pump to function properly. The name of the game in HPLC is repeatability. , 10 mL/min) to clear all the air from the pump and connecting tubing. 6. Filter all samples and standards 3. Otherwise, locate where the increased pressure is coming from. Replace PTFE frit in the purge valve. Vanquish Binary Pump C: Up to 700 bar (10,000 psi) and 10 mL/min. Pump & Maintenance Kits for HPLC Part Number: 01018-22707. Then wash them out with water and re-assemble them on the pump head. 1, 3, and 4. Label bottles correctly with bottle content,. 02-0. In other words is the system configuration comprising of the pump, injector/autosampler, column, and detector is appropriate for carrying out the analysis in. Retention. Make sure that you know which solvent is linked to which pump. 23 MPa. Our industry leading LC-40D X3 pump ensures precision and accuracy at all flow rates. In a low-pressure gradient (LPG) pump type, a proportioning valve is used to aspirate the desired solvent mix. 0Mb)Seal could have fluoropolymer O-ring energizer instead (black O-ring). Turn the handle counterclockwise, about 1/2-turn, to open the valve, and pull back on the plunger to remove air bubbles. Plunger indicator rods Show position of each pump head plunger. • Vacuum sensor – A sensor on the control board that monitors the vacuum in the system. Troubleshooting Strategy and Processes 5. The HPLC pump, often called a solvent delivery system, provides a precise flow of mobile phases of a specified composition to the column. L/mni for 5mni . ), prime system if necessary. Between samples when your compound is very sticky (apolar) to help clean the column. 1–1% solution by volume is sufficient. 4. The auto purge function was achieved by devising the mechanism of flow lines including the high-pressure 6-way valve and by modifying the solvent delivery unit check valve. Pump Types LPG pump type Eluent composition at the low pressure side => Before the pump head Eluent is composed through proportioning valves Eluent segments pass working and equilibration cylinder Up to four solvents can be mixed HPG pump type Eluent composition at the high pressure side => Behind the pump head UltiMate 3000 Series: Pump Series Operating Instructions Page I Declaration of Conformity (Original Declaration) Product: UltiMate 3000 Series Pump Types: LPG-3400AB, LPG-3400M and LPG-3400MB For stainless steel flow paths, proceed to Step 6; For PEEK flow paths, the cleaning procedure is completed. Press the EDIT/ ENTER key. HPLC Starter Kit G4201-68707 195 HPLC Starter Kit G4202-68707 195 HPLC System Tool Kit 196 Bottle Head Assembly 197 Solvent Cabinet 198. Tap the side of the check valve with a small wrench until small air bubbles come out with the liquid. The pump inlet port is closed to allow the pump to run with the gas ballast valve in the open position, which helps to purge the pump. The easy to use and economically designed LC-40D pump enables reliable HPLC chromatography in isocratic, binary, or LPGE modes. Before using an HPLC system, you should prime all of the lines in your HPLC pump. If your problem is. Changing the Purge Valve Frit/Purge Valve for Binary Pumps. However, even if the degassing system works properly, some air bubbles might stick in solvent lines or U/HPLC system fluidics. The new 1290 Infinity II LC power range has a high instrument efficiency, allowing you to run any HPLC and UHPLC method. 13), a precisely defined quantity of air (“the gas ballast”) is admitted into the pumping chamber of the pump. The high-precision P680 HPLC pumps are high-quality modules designed for HPLC analysis as part of a Dionex Summit HPLC system. 6:51. - after changing solvents. If 50% solvent A and 50% solvent B are programmed in the method, for example, the proportioning valve “picks” discrete fractions of solvent A and B to fill the piston chamber with the desired mixture. Purification Solvent Management. By the end of this video you will learn how to safely and quickly swap that suck. Sonication: ultrasonic baths as a stand-alone. When launching a long and continuous analysis sequence, for example 40 samples (analysis of each sample. A peristaltic pump (piezo pump) delivers fresh wash solvents to the fl ush port. Read Less. b. UltiMate 3000 Series: SD, RS, BM, and BX Pumps Operating Instructions Page I Declaration of Conformity (Original Declaration of Conformity) Product: Thermo Scientific Dionex UltiMate 3000 - Pump Types: ISO-3100SD, ISO-3100BMWe have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. The pump assembly includes a pump head with an active inlet valve which has an replaceable cartridge, and an outlet valve. 4. Equal functions for Right Pump found just below. 02 min SD (whichever is larger) of the set flow rate. Open the black purge valve on the front of the quaternary pump by turning it several times counterclockwise. Figure 1 Overview of the binary pump Purge valve Active inlet valve Mixer Outlet valve. 1 M is considered 'high', but all buffers should be flushed out) can damage the pump pistons, pump seals, injector. ⬇️ Get Affordable and Dope Lab Consumables Here ⬇️(No pun intended, unless you're a cannabis lab, then pun intended)to Connect with. 45 µm inert hydrophilic filter (avoid cellulose) and degas all eluents thoroughly, using sonication under vacuum. Achieve binary-like performance in your HPLC instrument. 6. Check for leaks and perform PM. 000, Ready, 0. 02 in. We have a HPLC Agilent 1100 with pressure problems. 5 mL/min d. try to locate source of particles (sample, eluents, pump mechanics, injection valve). (Prime each pump head separately. Sonication: ultrasonic baths as a stand-alone. View more in. 05 min is normal however the behavior of the method should be used to determine what is a normal variation. If adequate purging cannot be achieved with auto-purging or a suction tube does not contain liquid, for example, use the following procedure to manually purge with a syringe. 1. Preparing the Binary Pump 51 Algae Growth in HPLC Systems 52 Setting up the Pump with the Instrument Control Interface 54 Priming the Pump 64 Solvent Information 66. A = 100%). Purging the solvent pumps 1. After purging the pump heads, pu rge the ProFlow flow meter for about 10 minutes. off. Fig 2. During step 1, check that the solvent is freely moving through the solvent lines. Repeat twice after the cycle has finished after approximately 4 min. Make sure that the AVR7-3 inject valve is in the Purge position. For. However, in order to obtain a full understanding of the pump, Dionex recommends that you review the manual thoroughly before beginning operation. Treatment 1: Open a purge valve. 1. After purging is done click [Pump on] button / to deliver the solvent for at least 20 to 30min to rinse the flow line . Among the various technologies developed for chromatography,. 0046. 3:36. Manual - isocratic HPLC System G1380AA standard configuration • G1310A isocratic pump with solvent cabinet • G1328A manual injector (MI) • G1314A variable wavelength detector (VWD) with standard flow cell (10 mm path length, 14 µl volume, 40 bar maximum pressure) • G2220AA 2D-Value Solution ChemStation other modules that can be addedFailure to use fresh clean pickup filters in your HPLC may lead to total contamination of the flow path (column, pump, A/S and detector(s)). ) If system has check valve, loosen valve to allow air to escape. 1ml/min2 g. It is column chromatography. , 10 mL/min. 4411-EN Revision 2. Set a flow of 5 mL/min and purge for 5 min. Open the manual purge valve or use the purge command,. g. Press the EDIT/ ENTER key. Purging the Pump The quaternary pump will be purged by opening the purge valve. The 1290 Infinity II Flexible Pump combines highest performance, methods compatibility, and. Wash the system with isopropanol (excluding the column). Purge and condition lines A1, B1 using pathway 1, then A2, B2 using pathway 2 using a 50:50 composition to ensure both pumps are taking and delivering the same volume of HPLC flushing solvent. 액상미립자측정기에서의 purge는 측정완료된 샘플용액을 제거하기 위해 사용하는 것 같다. HPLC Pump – provides solvent flow and proportioning. Each channel comprises a pump assembly including pump drive, pump head, active inlet valve with replaceable cartridge, and outlet valve. Figure 2 shows how to perform the purge for an i-Series. 6. the solvent flow of the HPLC If equipped with a ballast valve check that the ballast valve on the rough pump is . Close the Purge Valve and flush the system. Then, turn it slightly past the initial resistance (check this). Purge valve Purge functionFig. Leak at fittings, purge valve, active inlet If there are still signs of a. Pump a 20% nitric acid/water solution through the pump for 3 minutes. Inside the purge valve is a small piece of polytetrafluoroethylene, called a PTFE frit. Documents & Downloads. 00 ml/min, turn the pump back ON and close the prime purge valve to allow the liquid to flow through solvent lines, through the degasser channel, through the pump. Open the purge valve by turning the knob on the front of the pump to the left until you feel some resistance. Solvent mixing: The proportionating valve (lower-front of image) allows the desired volume ratio of the different solvents to be drawn from the reservoirs. 2 Content 4 2. [][]Fill up & attach Labels of the solvent reservoirs properly. Confirm that the lever is in the INJECT position, insert the syringe all the way into the injection port, and quickly switch the valve to the LOAD position. . 3. Varian Modular Analytical HPLC Systems Figure 1. 5301 Stevens Creek Blvd. To purge the 2414 via Empower, use the Purge. An optional seal wash function is available for applications using Contacting Us Vanquish Pumps (VH-P10) Operating Manual Page 3 Contacting Us There are several ways to contact us: Ordering Information For ordering information or sales support for HPLC products, contact Solving Problems That Matter - Batteries. The descriptions in this manual apply to the LPG-3400XRS pump of the UltiMate™ 3000 series.